Photochemical production of ethylene from methionine and its analogues in the presence of flavin mononucleotide.

نویسندگان

  • S F Yang
  • H S Ku
  • H K Pratt
چکیده

In the presence of flavin mononucleotide and light, methionine is nonenzymatically oxidized to carbon dioxide, formic acid, ethylene, methyl disulfide, and ammonia. Tracer studies indicate that these products arise, respectively, from carbon atoms 1, 2, 3, and 4, methyhnercapto, and amino groups of methionine. It is suggested that the f&t photochemical reaction is the oxidation of methionine to methional, ammonia, and carbon dioxide, followed by the degradation of methional to ethylene and other products. The optimal pH for ethylene production by this reaction is about 8.5, and the reaction velocity is directly proportional to the concentration of flavin mononucleotide and methionine. Ethylene production is greatly inhibited by CL@, paramagnetic ions, and various phenolic compounds. Flavin mononucleotide can be replaced by riboflavin. Methional and cy-keto-y-methylmercaptopropionic acid are the most effective precursors of ethylene. Structural requirements for ethylene precursors were studied, and a mechanism accounting for ethylene production from methionine is described.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 242 22  شماره 

صفحات  -

تاریخ انتشار 1967